Background

This experiment explores the saccharification of Bloody Butcher corn using wheat koji to create an amazake-style beverage. The goal is to determine how effectively the starches in nixtamalized maize can be converted into sugars using non-traditional koji substrates.


Recipe

Ingredients

1 part Nixtamalized Bloody Butcher Corn

1 part Wheat Koji

3 parts Water

Equipment

Glass Jar

Fermentation Chamber

Instructions

  1. Combining
    Combine equal parts of the nixtamalized Bloody Butcher corn and wheat koji in a glass jar.

  2. Hydrating
    Add three parts water to the mixture and stir thoroughly to combine.

  3. Incubating
    Place the jar in a fermentation chamber and hold at 140°F (60°C) to initiate saccharification.

Notes and Observations

  • After being left at 140°F (60°C), the mixture did not saccharify as much as anticipated.
  • Despite showing good growth during cultivation, the wheat koji may not have produced a sufficient amount of amylase enzymes.
  • Alternatively, the starch within the nixtamal may not have been fully accessible for enzymatic breakdown.
  • Further experimentation is required to determine if maize starch is viable for amazake applications, which theoretically it should be.
  • A future control test will be conducted using rice koji to better analyze and isolate the issue.